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A helium flow (10 mL/min) was first used to flush the tube at 313 K for 15 min, removing all the remaining air inside.
DL-Cysteine in methanol (30 mg mL− 1, 200 mL) was added dropwise to the QD dispersion and mixed vigorously followed by centrifugation (10,000 rpm, 5 min), removing chloroform.
Mobility was analyzed by transferring five worms to the bacterial lawn of individual NGM/OP50 plates, incubating the plates at room temperature for five min, removing the worms, and imaging the worm tracks using a Zeiss MZ Apo stereo microscope equipped with a SPOT camera.
Cell layers were prepared for passage by adding 1 ml of 1 mg/ml Dispase (Gibco) to each well of the 6-well plate, incubating for 5 min at 37°C or 5 min, removing the Dispase, and washing the cells with 2 ml of DPBS.
Finally, we confirmed that the enzymatic activity measured in our assays with live cells was due to acid ecto-phosphatase(s) and not to released enzymes by incubating the same number of parasites used in the assay with the reaction medium at 37°C for 30 min, removing the parasites by centrifugation and testing for pNPP hydrolysis in this medium devoid of cells [ 64].
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The CC was immersed in aqueous rGO for 2 min, removed, and dried at 60 °C for 5 min.
Peels were loaded in the dark for 10 min, removed and floated on a dish of fresh buffer to wash off excess dye.
A hard bake step at 150 °C for 1 min removed surface cracks.
With this water, cook the shiitake for 30-40 mins, removing any scum from the surface.
Add the cockles or clams to the broth for 1-2 mins, remove and set aside.
Spin 2200 rpm for 5 min Remove supernatant.
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