Sentence examples for min protected from from inspiring English sources

CellTiter-Glo reagent was added to the wells (50 μL and 50-μL media) and incubated at room temperature for 10 min protected from light.

To this mixture, 5 μL of iodoacetamide (375 mM) was added, and the reaction continued for 30 min, protected from light, at room temperature.

After washing with PBS-T (6 times) TMB substrate (Medicago) was added and the signal allowed developing for 20 min protected from light.

EdU was detected by incubating cells with fresh Click-iT™ buffer for approximately 30 min, protected from light (Click-iT™ EdU Alexa Fluor® 488 Imaging Kit # C10083 from Invitrogen).

The plate was washed, incubated with streptavidin-HRP diluted 1 40 in OBB-PBS and spun at 1500 rpm for 3 min protected from light.

Subsequently, the cells were incubated with fluorescence-labeled secondary Alexa Fluor® 594 (Molecular Probes) at room temperature for 30 min, protected from light.

Samples were then incubated on ice for 10 min protecting from light.

The polyacrylamide mini-gel was run for 60 min at 25 mA protected from light.

The cells were incubated at 37°C for 10 min and protected from light.

In brief, initially reaction mixtures containing 25 μL CAT-containing samples and 25μL 40 μM H2O2 were incubated at room temperature for 30 min. Then 50 μL 100 μM Amplex Red reagent containing 0.4 U mL−1 horseradish peroxidase was added to each microplate well containing the samples and controls and incubated at 37 °C for 30 min under protected from light.

After 20 min incubation at RT with constant agitation, the plate was washed and incubated with substrate solution for 20 30 min at RT protected from light.