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Subjects were placed in a chamber for 3 min to explore the new environment, followed by a 3 min presentation of the CS while freezing was recorded.
Training consisted of a 10 min habituation to the training chamber with air, a 10 min presentation of odor 1 (1:36 odor:mineral oil actively streamed at 2 l/min), then 10 min of odor 2 (1:36 odor:mineral oil actively streamed at 2 l/min), with 60% ethanol.
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Each session lasted for approximately 1 h, beginning with a 20 30 min presentation on the topic of the day, followed by a related exercise (e.g., drawing a life chart or compiling a list of potential triggers for relapse).
On the last day of the workshop, each group gave a 15 20 min presentation on its teachable unit.
Upon the 12-min presentation of a visual distracter (flashing houselight, 0.5 Hz) during task performance, animals initially developed a significant response bias to the left lever in the first 6-min distracter block, reflecting a reduction of attentional effort.
A larger and more pronounced glucose response occurred during a 1-min presentation of a novel object, another presumably stronger arousing stimulus (C, D).
On day 1, the mice were allowed to become familiar with the test situation by placing them in a clean cage with bedding for 3 min, followed by a 2-min presentation of water (100 μl) on a cotton bud inserted through the lid.
Figure 4 shows a picture taken during 1 of the 5 min presentation, in the speech session.
Duration of sniffing was measured over a 3 min presentation.
Recording sessions started with 5 min of adaptation to the background, followed over 35 min with presentation of multiple stimulus pairs with pseudorandomized order for test and reference stimuli.
Rats received 30 CS presentations (white noise, 30 s, 80 dB, 5 s inter-stimulus interval) at 5 min after placement in context B. Rats were returned to their home cages at 5 min after presentation of the final CS.
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