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In comparison, the Xpert MTB/RIF system enables hands-on time of less than 3 min per specimens being of advantage only when handling a small number of samples.
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The overall imaging time was 7.4 min per specimen.
Hands-on time to process specimens and perform fully automated real-time PCR with Xpert MTB/RIF was less than 3 min per specimen with a turn-around time (TAT) of less than 2 hours.
Typically, the spiral consisted of 20 complete rotations, images were acquired every 1° at 60 frames per second (fps), and the total acquisition of 7200 images took less than 2 min per specimen.
Running on a single CPU core, this required an average per-specimen running time of 4 h for preprocessing, 30 min for segmentation, and 4 min for model fitting and parameter estimation.
Total effective dose was 24.86 mSv per specimen.
The operative time is not any longer for MCE but processing the specimen takes 10 25 min per micrographic layer.
Cement specimens were sterilized in water under UV light (having a peak wavelength of 254 nm, 45 min per side) prior to being immersed in the liquid, 20 mL per specimen (corresponding to a liquid-cement-volume ratio of 60 [ 5]), and kept at 37°C until testing in allocated experiments.
Extraction time: 84 min per shift.
Solution access was limited to 30 min per session.
Testing occurred over 2 days (60 min per day).
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