The measurements performed here are inherently fast, averaging about 3 min per sample.
The total assay time is 5 min per sample, including the regeneration step.
After significant variables optimization, extraction procedure times were 7 min (7 min per sample) and 70 min (12 min per sample) for enzymatic extraction assisted by pressurisation and microwave energy, respectively.
Additionally, a high sample throughput was achieved after optimization due to a total runtime of maximally 40 min per sample.
Tandem Reversed Phase HPLC was applied for very fast quantification of both impurities and active (biotin binding) avidin within 4.25 min per sample.
The sequential methodology, which can be performed simultaneously on three samples, requires less than 20 min per sample for fusion and separation.
Highly satisfactory separation of HMW-GS and quantification of ratio of HMW- to LMW-GS were achieved in less than 4 min per sample using a modified HPLC gradient.
In conclusion, because of the lower degree of variation, the numbers of spermatozoa analyzed, and the speed of analysis (∼1 min per sample), QualiSperm™ appears to be a suitable instrument for routine work, provided it maintains stability and is available at an affordable price.
A typical analysis time is 1 2 min per sample.
Count data were acquired for 1 min per sample unless otherwise stated in the text.
The sample preparation protocol was time efficient (~5 min per sample) and required only 10 mg of sample powder.
