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Owners spent 10.0 ± 0.8 min per egg on parasitism-related behaviour (range = 25.4 min per egg), and intruders spent 7.1 ± 0.8 min per egg on parasitism-related behaviour (range = 17.1 min per egg).
Solo females spent 11.3 ± 0.6 min per egg on the four parasitism-related behaviours: drum, probe, hostfeed and oviposit.
The time required to complete parasitism of one egg varied between females with some spending extended periods on a single egg and others parasitising multiple eggs in rapid succession (range = 26.8 min per egg).
After six days at the non-permissive temperature, these animals showed a significantly lower rate of egg laying, about half of normal and ovulated much more slowly than controls, requiring an average of 27 ± 6.1 min per egg compared to 7.6 ± 3.6 min in controls (Table 2).
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The experimental design used previously (i.e. six eggs per dose group, scoring of 1000 cells per egg) was confirmed.
Feed intake (FI), egg production (EP), egg weight (EW), egg mass (EM), feed conversion ratio per dozen eggs (FCRD) and feed conversion ratio per egg mass (FCRM), yolk, eggshell, albumen and excreta dry matter (EDM) content were evaluated.
For all microinjection experiments, embryos were collected within 5 min of egg laying.
Therefore, a standard sperm to egg ratio of 1 × 105 sperm per egg is recommended for fertilization in Atlantic cod.
We measured the number of egg masses produced and the number of eggs per egg mass.
At a density of 1.6×104 packed eggs per µl (n = 1), embryonic cell isolation produced 3.8±1.8 cells per egg.
For egg size, we measured the area (mm) of 5 randomly selected eggs per egg mass.
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