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The reaction mixtures were incubated for 20 min on ice.
For retina staining, eyes were dissected and fixed in 4% PFA for 30 min on ice.
Samples were mixed by pipetting, and after 1 min on ice, centrifuged at 1000 × g.
For RAD51 staining, cells were pre-extracted in the CSK buffer for 4 min on ice.
Firstly, the cells were lysed in 100 µL lysis buffer for 15 min on ice.
and incubated for 30 min on ice.
BSA for 30 min on ice.
The suspension was then incubated for 30 min on ice.
Permeabilization was by 90% methanol for 30 min on ice.
Extraction was performed for 30 min on ice.
Then, cells were incubated for 30 min on ice.
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