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In initial sessions, food rewards (chocolate pellets) were delivered to the reinforcement tray every 20 s for 10 min on day one and for 20 min on day 2. Pellet delivery was signaled by the brief onset of a buzzer.
Irinotecan was administered at a dose of 300 mg m−2 infused over 30 min on day one, repeated every 3 weeks.
Sleep duration (min/30 min) on day 3 21.5°CC; permissive temperature), days 4, 5 28.5°CC; non-permissive temperature) and day 6 21.5°CC; permissive temperature) are plotted (colored line, split-GAL4 line in combination with pJFRC124-20XUAS-IVS-dTrpA1 (attP18 ); black line, represents +/dTrpA1; gray line, split-GAL4/+).
In the IP arm, cisplatin, 60 mg m−2, was administered intravenously over 60 min on day 1 and irinotecan, 60 mg m−2, was administered intravenously over 90 min on days 1 and 8. Prophylactic granulocyte colony-stimulating factor (G-CSF) was not administered in this arm.
Treatment included 1,000 mg/m(2) intravenous gemcitabine over 100 min on day 1, 10 mg/kg intravenous bevacizumab on day 1, and 100 mg/m(2) oxaliplatin given on day 2. Cycles were repeated every 2 weeks.
All patients also performed SMBG every 15 min on day 10.
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Forty eligible patients received carboplatin (area under the plasma curve [AUC] 4 mg/ml-min) on day one, while bevacizumab (10 mg/kg) and irinotecan (340 mg/m(2) for patients on CYP3A-enzyme-inducing anti-epileptics [EIAEDs] and 125 mg/m(2) for patients not on EIAEDs) were administered on days 1 and 14 of every 28-day cycle.
2) IPC+I/R group: Rats were prepared in the same way for 4-vessel ischemia, but instead of 15 min, the first, preconditioning, ischemia lasted 3 min. On day 3, a second global ischemia was induced for 15 min, and three days later (day 6) the rats were sacrificed under halothane anesthesia.
To acclimatize the mice to the apparatus, mice were run gently downhill on a treadmill inclined at 15° for 10 min at 9 m/min on day 1, then 10 min at 10 m/min on day 2 and 10 min at 10 m/min on day 3. On day 4, the mice were run for 10 min at 10 m/min, at which point the speed was increased within a few seconds to 16 m/min.
On Day 1, the rats were placed inside the arena in groups of two or three for 10 min. On Day 2, the rats were placed inside the arena one at a time for 5 min. Two objects (a blue beer can and a large ceramic figurine of Snoopy, 15 cm high from Peanuts, a comic strip by Charles M. Schulz) were placed 10 cm away from the middle of the left and right walls, respectively.
Activity was recorded for 90 min. On Day 2, mice were habituated to the Open Field chamber for 60 min. They were then injected with saline (10 ml/kg, s.c).
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