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A profound prolongation of reaction times induced by hypoglycemia in both groups persisted even after 30 min of subsequent euglycemia.
Figure 6b d shows records of APs obtained at a stimulation frequency of 1 Hz under conditions of normal extracellular pH (left panels) and after 5 min of subsequent metabolic acidification (right panels) of cells patch-clamped using either the BAPTA (Fig. 6b,e) or the EGTA-BAPTA solution (Fig. 6c,d).
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Dopamine, quipazine and VUF10166 were partial agonists at wild type 5-HT3A and 5-HT3AB receptors, with quipazine and VUF10166 causing a long-lived (>20 min) inhibition of subsequent agonist responses.
In an attempt to maximise ecological validity, we have not analysed the first of the 18 samples, nor the first 5 min of any subsequent sample.
During the first 2.5 min of a subsequent exposure to 5 μM 6-OH-BDE-47 (n = 9), the release frequency was enhanced from 1.0 ± 0.3 to 13 ± 5.3 events/min (p < 0.05).
In the first three groups, serum PK samples were collected at 1, 4, 10, 24, and 72 h after the first infusion and then pre-dose and 60 min after start of subsequent infusions at weeks 1 3 and 6 11.
Consistent with this, under our conditions (45 min of CD3 engagement), subsequent adhesion of T cells to ICAM-1 following removal from anti-CD3 antibodies was not altered (Figure S1B), indicating that the effect of CD3 activation on T cell migration was not due to changes in cell adhesion.
After 20 min of shaking and subsequent centrifugation, the protein concentration of nuclear extracts was determined (Bio-Rad).
Whole blood anticoagulated with PPACK was immunolabelled within 5 min of phlebotomy for subsequent flow cytometric analysis.
Five genes (0.08% of the genome) were up-regulated, and seven genes (0.11%) were down-regulated after 15 min of fixation and subsequent centrifugation (Table 1).
First, we have observed the effect in sequential treatments with control medium (30 min) followed with 30 min of CCL19 and subsequent CK1 inhibition (30 min) while imaging MEC1 migration in standard glass-bottom plates.
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