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Enhanced glucose clearance was achieved when plasma NEFA was reduced by NA, despite lower insulin concentration (70.0 vs. 97.9 ± 13.4 μIU/mL) and a tendency for smaller insulin response area under the curve during 180 min of sampling [7,646 vs. 12,104 ± 2,587 (μIU/mL) × 180 min], reflecting an increased response to endogenous insulin.
The reduction of plasma NEFA concentration led to significantly greater glucose clearance rate (1.9 vs. 1.2%/min) and to decreased glucose half-life (37 vs. 58 min), time to reach basal concentration (81 vs. 114 min) and glucose response area under the curve during 180 min of sampling [6,942 vs. 10,085 (μIU/mL) × 180 min].
The samples were analyzed in a standardized manner within 2 min of sampling.
Following centrifugation, the supernatant was transferred to a 15-mL polypropylene falcon tube and divided in 0.5-mL aliquots, placed on dry ice within 30 min of sampling and transferred to −80 °C for storage within 60 min of sampling.
Blood samples were collected in EDTA tubes and centrifuged at 2000 g for 10 min within 30 min of sampling.
No B. anthracis was identified on any of the SSSB plates during the 20 min of sampling.
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The upper and lower bounds depend on max and min values of sampled path loss data.
A preconcentration factor of 20 and a detection limit (3sb) of 2.1 ng L−1, along with a sampling frequency of 28 h−1 were achieved with 1.4 min of sample loading time and with 2.8 mL sample consumption.
The resulting DNA duplexes could be visualized trapped at the LFD strip test line within 5 min of sample exposure.
Fig. 3 Plot of particle sizes (μm) versus ground time (min) of samples S4, S5, S6, S7, and S8.
Disintegration was performed at 4000 rpm in three cycles consisting of 2 min of disruption and 2 min of sample cooling on ice.
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