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We have reported that 5 min of restraint reduced lordosis behavior of ovariectomized rats hormonally primed with estradiol benzoate.
The second occurred at the same time of day but followed 20 min of restraint stress in a Plexiglas tube.
Lordosis behavior of rats primed only with estradiol benzoate declined after the 5 min of restraint while allopregnanolone prevented this decline.
While circulating ACTH levels increased after 5 min of restraint, levels were not greater in chronic stress animals compared to controls until following 20 min.
(b and c) There were no differences between vehicle and TAM groups in plasma CORT levels before or at various time points following 30 min of restraint stress or immediately following 15 min in a novel cage (P>0.05).
Stressed samples were obtained from separate individuals (n = 5) after 60 min (63.43±0.59 min) of restraint in an opaque cloth bag.
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Rats in group CT were handled in the same manner as those in group ST and group SC but were returned to their home cage for 30 min instead of restraint.
We assume the non-facilitation of the general LTD response of our sham or EMF exposure treated groups to be derived from the initial CORT elevation during the first 30 min of tube restraint within the ED.
Our results showed a positive association between Pb and the stress-induced response elicited after 45 min of handling and restraint regardless of colony location.
Data inspection suggested that, whereas mice from the three groups showed similar circulating levels of CORT under basal conditions, immediately after restraint and 120-min following the beginning of restraint, H-CORT mice showed higher CORT response 60-min after the beginning of restraint (see Fig. 5b).
We designed a study to examine the effects of an acclimatization protocol (5 days of restraint, 60 min per day) on the emission of 22-kHz ultrasonic vocalizations and performance in a forced swim test (FST).
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