Sentence examples for min of preliminary from inspiring English sources

PCR assays (each of 35 cycles) were performed as follows: 5 min of preliminary heating at 94°C, 94°C for 40s, 50°C (for GABA A α1/β3 /55°C (for actin) for 40s, 72°C for 40s, and 72°C for 10 min.

PCR reactions were performed using the following protocol: 3 min of preliminary activation of Taq polymerase at 95°C were followed by 32 (34 for nuclear genes) three-step cycles of denaturation at 94°C (30 sec), annealing temperature specific for primer (30 sec; see Additional file 1: Table S1) and extension at 72°C (70 sec), and a final extension at 72°C (10 min).

Blood glucose level was significantly elevated due to dextrose perfusion in both AHG + Isch and AHG + RIPerC groups compared to the corresponding NG group from at least the 15th min of perfusion [Table  1, preliminary results showed that acute hyperglycemia developed in 5 min (17.2 ± 1.8 mM, n = 4)].

After a median time of 14 min the preliminary histological diagnosis was available.

Similarly, at the later time point (120 min), our preliminary observation of the thin sections showed that the MTs are at a very close proximity to the SPB in the hrs1Δ samples (For example, see Figure S1C) indicating that the rMTOC structure is completely missing, rather than delayed, in the absence of Hrs1.

After 90 min the reaction was complete (as determined by NMR of preliminary reaction runs).

PCR amplification steps consisted of preliminary denaturation step at 95 °C for 3 min, followed by 34 cycles at 95 °C for 15 s, at 57 °C for 15 s, and at 72 °C for 1 min.

In a set of preliminary experiments, where the testing period lasted 10 min, observations of tail rattling and wrestling behavior were included in the ethogram.

In preliminary studies, 3 min of upper arm blood flow occlusion at suprasystolic pressure was found to be the upper limit of tolerability.

While this can be administered intralesionally, a number of preliminary topical trials have been performed, applying mitomycin C for 2 4 min directly following keloid excision.

It was found from a preliminary test that 10 min of resting time is enough for all sample solutions to be completely relaxed and to be thermally equilibrated.