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Both have narrow peaks, which occurred within 8 min of one another in LST.
However, the redistribution to PM is supported by one low-resolution image (see Figure 4A, 1 min vs. 0 min) of one cell without quantifications.
PCR cycling conditions were 10 min of initial denaturation at 95°C followed by 35 cycles of 15 sec denaturation at 92°C and 1 min of one step annealing/extension at 60°C for MMP-1 or 62°C for MMP-3 (ABI Prism® 7900 HT Sequence Detection System, Applied Biosystems).
The PCR was carried out in the reaction mixtures according to the provided protocol of rTaq and amplification was under the following conditions: 95 °C for 5 min of one cycle, followed by 35 cycles of 30 s at 95 °C, 40 s at 58 °C and 50 s at 72 °C, and a final stage of 5 min at 72 °C.
At 12 00 a.m. the subjects received a lunch, which they consumed within 30 min of one of two randomly assigned treatments: control lunch or the lunch supplemented with 1,030 mg red pepper equivalent to 80,000 Scoville Heat Units (SHU) of capsaicin (Cayenne, Solaray, Park City, UT, USA).
Repeated aggressive interactions between two individuals were treated as a single event if they occurred ≤3 min of one another.
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Applying the extended MST approach to the complete Bootstrap network took < 3 min on one processor of an Intel Core2Duo with 2.4 GHz.
Additionally, the average distance between the communities and the nearest hospital with an ED was 12.5 ± 9.3 km, corresponding to 17.6 ± 10.2 min of one-way travel.
Other studies show similar upregulation of VEGF mRNA in chronically electrically stimulated rat skeletal muscle [ 13, 14], and in human vastus lateralis following 45 min of one-legged knee extension exercise [ 15].
The average distance between the communities and the nearest HC provider, either a local outpatient clinic or a hospital, where patients could receive visits and examinations, was 7.5 ± 6.2 km, corresponding to 12.2 ± 8.2 min of one-way travel.
We have previously demonstrated that VEGF mRNA is upregulated in rat gastrocnemius muscle following 1 hour of acute submaximal treadmill exercise [ 10], in dog gastrocnemius muscle following 1 hour of electrical stimulation [ 11], and in human vastus lateralis following 30 min of one-legged knee extension exercise at 50% maximal capacity [ 12].
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