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All mice survived the 240 min of mechanical ventilation.
After 20 min of mechanical ventilation, there was a dramatic increase in lung water content and pulmonary permeability (p < 0.01 vs. other groups).
BALF cells from control, non-ventilated mice and from mice subjected to 60 min of mechanical ventilation consists of >85% alveolar macrophages (data not shown).
Three mL of sodium chloride 0.9% were added, followed by 4 min of mechanical homogenization with a Stomacher 80 Biomaster (Seward, Worthing, UK).
Clearly, HBO (60 min of mechanical ventilation with pure O2 at 1.5 atmospheres of ambient pressure was shown to efficiently decrease ICP in patients with traumatic brain injury (TBI) [115].
Similarly, after 240 min of mechanical ventilation with normal VT, WT, and Tpl2-/-, the mice had no differences in all indices of lung injury studied, inspiratory capacity (Figure 2), arterial oxygen (Figure 3), and concentration of proteins (Figure 4) and IL-6 (Figure 5) in BALF.
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In contrast, in the majority of previous studies the P-V curves at ZEEP were recorded after a 15 30 min period of mechanical ventilation at ZEEP, allowing complete lung de-recruitment to take place.
Second, we performed dose-response experiments (four increasing doses of morphine) with a time-dependent (30, 90, 180 and 360 min) evaluation of mechanical threshold, while Kroin et al.
To confirm the receptor-dependent effect of morphine and that an opioid-receptor-dependent inhibition of the Ehrlich tumor-induced hyperalgesia was being observed, mice were treated with naloxone (1 mg/kg, i.p). 1 h before morphine (10 mg/kg) treatment, and after additional 45 min, measurements of mechanical) and thermal) hyperalgesia were performed.
In C2C12 myotubes subjected to a 30 min lapse of mechanical stretch, EGR1 protein content increased immediately after the application of stretch, peaked between 3 and 4 hours after stretch and diminished rapidly in the next 2 hours (Fig 1a and Fig 1S).
After 12 min of asphyxia, mechanical ventilation was restarted and CPB was started.
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