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Wistar albino rats through clamping hepatic artery, portal vein, and bile duct, were subjected to 45 min of hepatic ischemia followed by 60 min reperfusion period.
Rats were fed a Lieber DeCarli diet for five weeks to allow for development of AFL and were then subjected to 90 min of hepatic ischemia and 5 h of reperfusion.
Following 30 min of hepatic ischemia, all livers were reperfused.
In the I/R group, the rats were subjected to 60 min of hepatic ischemia followed by 60 min of reperfusion period.
Our previous published work has shown that 90 min of hepatic ischemia followed by reperfusion caused hepatocellular injury in a time-dependent fashion, as demonstrated by plasma ALT level [ 11, 14].
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After 60 min of partial hepatic ischemia, the clip was removed to recover hepatic reperfusion for 24 hours.
After 60 min of partial hepatic ischemia, the clip was removed to initiate hepatic reperfusion and the abdominal cavity was closed with a 4-0 sutureuture.
After 40 min of partial hepatic ischemia, the vessel clip was released to initiate hepatic reperfusion.
After 90 min of partial hepatic ischemia, the clamp was removed and reperfusion was resumed.
Sixty patients undergoing whole-body PET/CT 60 min post-injection of FDG first had dynamic PET imaging for 30 min with measurement of hepatic and splenic FDG clearances using Patlak-Rutland analysis.
The glucose infusion rate in FGF21-Tg mice is statistically different (p<0.05) from WT mice at all points after t=0 min. (D ) Rates of hepatic glucose production (upper panel) and whole-body glucose disposal (lower panel) in WT (n=6) and FGF21-Tg (n=3) mice during the basal and steady-state periods of the clamp.
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