Sentence examples for min of final from inspiring English sources

PCR amplification was performed after an initial denaturation of 3 min at 94°C, followed by 40 cycles of 45 s at 94°C, 45 s at 54°C and 1 min at 72°C, and finally 10 min of final elongation at 72°C.

PCR conditions for the ACT1 gene amplification were as follows: 3 min of initial denaturation at 94°C, followed by 35 cycles of 45 seconds at 94°C, 45 seconds at 56°C, 1 min at 72°C and terminating with 7 min of final extension at 72°C.

GPD1 and IGS1 of the other strains were amplified using the primers GPD1F/GPD1R [68] and IGSF/IGSR [15] under the following conditions: 3 min of initial denaturation at 94°C, followed by 35 cycles of 45 seconds at 94°C, 45 seconds at 63°C (GPD1) or 60°C (IGS1), 1 min at 72°C and lastly, 7 min of final extension at 72°C.

After 30 min of final washes in PBS-T and 10 min in PBS, sections were mounted with SlowFade Gold (Invitrogen, Burlington, ON, Canada).

The thermocycling conditions were 95 °C for 5 min for denaturation; then 39 cycles of 30 s at each temperature of 95 °C, 66 °C and 72 °C; and 5 min of final extension at 72 °C.

Subsequently, 35 cycles of denaturation at 94°C for 20 sec followed by annealing for 20 sec at 56°C and extension for 30 sec at 72°C and 20 min of final extension at 72°C.

PCR parameters were 95 °C for 5 min of initial denaturation, 95 °C for 30 s of denaturation, 59 °C for 2 min of annealing, 72 °C 4 min and 72 °C of 10 min for final elongation on Eppendorf Mastercycler personal, Germany.

Cost-effectiveness is high, with 15-30 minutes/transect from field data collection (< 10 min) to computation of final percent covers 10-200 min).

It took about 2 min for final setting of the reaction pressure and reaction time will be considered as zero.

For concentrated nanofluids, stock solutions of gold nanoparticles synthetized by hydroquinone method were centrifuged at 6,000 rpm for 30 min for final concentrations of 1 mg/ml.

When the Pt loading time were 2.5, 5, 10, and 20 min, the percentages of final dissolved As V) in system were 77.3, 83.9, 88.1, and 85.9, respectively.