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Following an additional 45 min of color development, the release of inorganic phosphate was measured as absorption at 660 nm.
After 10 min of color development at room temperature, the absorbance was measured at 540 nm with a Micro-Reader (Molecular Devices, Orleans Drive, Sunnyvale, CA, USA).
During all the experimental phases, the percentage of time spent in each side of the tank and distance traversed was analyzed within a) 120 min prior to red color presentation time, in 30-min intervals; b) during 5 min of color presentation prior to food (or corresponding period when red color was not used) and c) 60 min after food was administered (Fig. 1A).
Each spectrum was measured after 30 min of color development.
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After stirring for 3 min, the degree of color development was measured at 500 nm.
Following the addition of 1 ml of color B reagent, the sample was incubated at 37 °C for 10 min.
After an induction period of 30 min, the color of the solution changed from brown to green, which confirmed the polymerization of aniline in conducting form.
During further stirring for 30 min the color of the solution changed to brown, which indicated the formation of the Ce-POBC-Co single-source precursor.
Alumina powder (60-325 mesh BSS) was added to aqueous Ag8,7 QC solutions, and the mixture was stirred for 5 min. Color of the alumina powder changed to orange, indicating that the QCs of Ag7,8 got loaded on alumina.
After 10 min, the color of the AgNO3-containing solution clearly turned to brown, which indicated the formation of AgNPs in the solution.
This was followed by centrifugation for 5 min; the color of the supernatant was determined using spectrophotometer at wavelength 480 nm.
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