Sentence examples for min of cold from inspiring English sources

Transmission electron microscopy revealed significant physical cellular damage after 2 and 4 min of cold plasma treatment.

All hearts were subjected to 30 min of cold ischemia (+4 °C) and 6 h of RP before explantation.

Time from cross clamp to reperfusion was 569 ± 28 min, of which 470 ± 24 min of cold and 98 ± 14 min of warm ischemia, respectively.

All treated mice received 5 min of cold plasma treatment.

After 30 min of cold immersion, the hand and foot were taken out of the water tanks and patted dry with a towel.

We demonstrated that radiolabeled rCR2 could be u­sed to detect complement activation and, in particular, C3d expression on the transplanted heart, which had undergone 35 min of cold ischemia followed by 24 h reperfusion.

Briefly, nuclei were resuspended in lysis buffer (Tris HCl 50 mM pH 8, MgCl2 5 mM, KCl 25 mM containing HCl 0.2 N, 1 mM NaF, 1 mM Na3VO4, 2.5 mM sodium butyrate and Roche's EDTA-free protease inhibitor cocktail), and incubated on ice for 30 min. After centrifugation at 11,000 g for 15 min, 1 ml of cold acetone was added to the supernatant and placed at −20°C for 4 h.

After 20 min, 40 mL of cold, sterile water was added and mixed.

After 15 min 1 ml of cold, distilled water was added to the tubes, which were then centrifuged at 2 8°C.

After 30 min, 2 ml of cold methanol was added, and incubated over night at −20°C to precipitate the proteins.

The samples were shaken for 10 min; afterwards, 1 ml of cold chloroform containing 0.1% BHT was added to these samples and then shaken for another 10 min.