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The mean adsorptive clearances after 30 min of circulation are represented as a function of tested molecules ranked according to their respective molecular weight (Fig. 4).
Here, k is the slope of decay, t is the time, V is the plasma reservoir volume, and CL 30) is the mean adsorptive clearance over 30 min of circulation.
The mean adsorption clearance for each mediator was simulated using a model of monoexponential decay (limited to the first 30 min of circulation), employing the two equations: CB 30) = CB 0) e k t and CL 30) = k × V, where CB 0) and CB 30) were respective concentrations in the plasma reservoir at t0 and t30, respectively.
After 30 min of circulation, the mice were perfused with PBS containing 1% BSA, and their organs were collected.
After 100 min of circulation time, the mice received an intravenous infusion of avidin over a period of approximately 15 min to induce rapid blood clearance of the liposomes.
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Significant reductions in liver accumulation were observed for different formulations of the layered architectures within the first 30 min of systemic circulation.
Leakage of infused radioactive albumin during the 3 min of its circulation time must be regarded as insignificant, and has been analysed before [35].
After imaging and after two min of beads' circulation the rats were sacrificed and the organs as well as the tumours were removed [17].
Electron microscopy of sections incubated after 10 or 20 min of HRP circulation confirmed the paravascular location of the reaction product, which was also dispersed throughout the extracellular spaces (ECS) of the adjacent parenchyma.
Hoechst dye was injected intravenously into Tie-2 tTA//TRE-claudin-1 C57BL/6 mice and single transgenic littermates at day 50 after induction of EAE, after 30 min of dye circulation, brains were removed, and immunofluorescently stained for claudin-1 and for infiltrating leukocytes with the pan-leukocyte marker CD45.
Samples were taken before and 15, 30, 60, 120, and 180 min after commencement of circulation.
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