The precipitate containing WPs was centrifuged at 6000 rpm for 6 min followed by 3 min of centrifugation at 10,000 rpm (Eppendorf, Centrifuge 5430).
Tubes were shacked for 30 s at 22 °C and bacteria were harvested by 10 min of centrifugation at 3000 g (Eppendorf 5415 C centrifuge).
The protein lysate was incubated on ice for 30 min followed by 20 min of centrifugation at 13 000 r.p.m. in an Eppendorf tabletop centrifuge (Hamburg, Germany) at 4°C.
The optimized condition of the developed method was: 20 min of centrifugation at 9000 rpm using the acid mixture 1.0 mol L− 1.
The optimized method includes12 mL of extractor solvent, 5 min of vortexing time, 5 min of centrifugation time and 12 h of freezing time.
After 8.5 min of centrifugation, sedimented phase (surfactant-rich phase) was withdrawn by microsyringe and injected into the HPLC system for analysis.
The optimized USAEME procedure used 200 μL of chloroform as extraction solvent, 10 min of extraction with no ionic strength adjustment at 25 °C and 5 min of centrifugation at 4000 rpm.
For 10 mL of water sample, the optimized USAEME procedure used 200 μL of chloroform as extraction solvent, 15 min of extraction without ionic strength adjustment at 25 °C and 5 min of centrifugation at 4000 rpm.
Crude protein extracts from flies were prepared by using the polypropylene pestle grinder (Sigma) and the enzyme assay buffer (50 mM Tris acetate, pH 8.0, 0.1 mM EDTA and protease inhibitors) followed by 10 min of centrifugation at 13,500 × g.
The optimum values of the factors that influence the capacity to scavenge DPPH and ABTS+ radicals or to inhibit β-carotene bleaching were 3 extractions at 25 °C for 0 min in the water bath (the rest of the extraction process included 1 min of high-speed homogenisation and 20 min of centrifugation).
Supernatants were thrown after 5 min of centrifugation at 13,000 rpm.
