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Bats were sampled as previously described [17], typically within 5 10 min of capture, and then released.
Adults were released within 10 min of capture.
For the remaining immune traits, blood sampling occurred within 10 140 min of capture.
Plasma IgY and Hp were obtained from blood samples taken within 3 min of capture and handling.
Blood samples were collected within 5 min of capture and on the following schedule: baseline, 30 d, 60 d, and 90 d.
All fishes were euthanized with an overdose of MS-222 (Sigma) within 2 min of capture, and the brain and gonads were dissected immediately.
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Under unstimulated or lightly-activated conditions, up to 90% of adherent leucocytes are known to detach into the bulk flow within 15 min of initial capture (Wojciechowski & Sarelius, 2005).
For each experiment, 30 tests of channel selection were performed with data of the two following minutes to the 4 min of the captured data.
Area under the curve (AUC) is measured from 64 96 min to capture the entirety of the glucose-stimulated insulin response curve.
Renal PCD CT images were acquired every 2 s for 5-6 min to capture the wash-in and wash-out kinetics of the contrast agents.
Blood samples for hormone determination were kept on ice until centrifuged (3,000 rpm for 10 min) the same day of capture, and plasma was frozen and stored at −80°C for radioimmunoassay (RIA).
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