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Each intervention was preceded by 5 min of baseline haemodynamic data collection.
After informed consent, participants completed 45 min of baseline assessments, and then completed a series of 12 identical, 5-minute exposure trials with inter-trial breaks.
During 40 60 min of baseline monitoring, mean brain and core body temperatures did not increase more than 0.2 ± 0.1 °C.
After 2 min of baseline measurement, the upcoming task was introduced and subjects were allowed to prepare themselves for 2 min.
To investigate whether the increase of (R -11C]verapamil bR -11C]verapamilng and after treatment with tariquidar (60 to 140 min of brainine scan) influptake the moduringtcome pandmeters, the finafterdel was applied treatmentrst 60 min of the baseline (i.e., excluding the data acquired during and immediately after tariquidar administration) and the post-inhibition scan only.
Additionally, the model was applied to the human data set, and the influence of including or excluding (R -11C]verapamil bR -11C]verapamilons during and after the tariquidar administration (40 to 120 min of brainine sconcentrationsduringtcome pandmeters wafteraluathe.
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Each subject was placed in the rectangular box for a total of 8 min. The first 2 min consisted of baseline exploration in the absence of the CS (pre-cue period).
The acute stress protocol was as follows: after a 30-min rest period following cannulation, a 5 min period of baseline blood pressure was carried out, together with a resting saliva sample, and blood sample.
Changes were quantified by normalizing and averaging EPSC amplitudes during the last 5 min. of the experiments relative to the 5 min. of baseline prior to LTP induction or drug application.
After a 5 min period of baseline recording from 4 of such neurons, riluzole (80 µM) was locally applied onto the DRG.
After a 20 min period of baseline recording, LTP was induced using either a high-frequency stimulation protocol (two trains of 100 Hz stimulation, each 1 s in duration and separated by 10 s) or a low-frequency stimulation protocol consisting of 900 stimulation pulses delivered at 5 Hz.
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