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The two experimental periods, applied in random order and with allocation concealment, were separated by 30 min of basal ventilation.
After 5 min of basal recording of arterial and left ventricular pressure, 1 and 15 μg/kg of dobutamine were administered intravenously in a random sequence.
The two experimental periods were applied in random order according to a computer generated randomization list, and they were separated by 30 min of basal ventilation.
Blood samplings were collected each 10 min during the last 30 min of basal period and each insulin infusion step of the clamp to determine glycemia, insulinemia, plasma FFA, TGs, and glucose isotopic enrichment in H.
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Insulin secretion by untreated (control) islets estimated as integrated area under the curve (AUC/ 20 mins) above basal during three pulses of 27.7 mM glucose separated by 20 min periods of basal (washout) perifusions were 2950 ± 585 pg, 5185 ± 1258 pg, and 2410 ± 921 pg, a greater response (P < 0.03) being obtained during the second glucose challenge.
Plasma triglycerides were determined at −30 and 0 min of the basal infusion period.
Isotopic steady state was achieved during the final 30 min of the basal period and the final 30 min of the hyperinsulinemic clamp study.
Flow measurement of glucose turnover was measured in the final 20 min of the basal period (calculations for times −20, −10, and 0).
Isotopic steady-state conditions were achieved during the final 30 min of the basal period and stages 1 and 2 of the clamp procedure.
Blood samples were obtained during the final 30 min of the basal and insulin infusion periods to determine plasma glucose specific activity and insulin concentrations.
Basal and insulin-stimulated nonoxidative glucose Rd was estimated by subtracting the rate of glucose oxidation from the total Rd during the last 30 min of the basal period (−30 to 0 min) and clamp (150 180 min).
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