Suggestions(4)
Similar(58)
hESC lysate for each time point (6 mg) was digested, followed by stable isotope dimethyl labeling, whereby the non-stimulated hESC (0 min) was labeled with light dimethyl labels, the 5 min time point with intermediate labels, and the 15 min time point with heavy dimethyl labels.
Cells that flashed fewer than 10 times over 30 min were labeled as 'infrequent,' whereas those flashing more than 10 times were categorized as 'frequent'frequent
For the last 15 min, cells were labeled with 35S-methionine.
After cooling for 5 min, slides were labeled with antibody using the Ventana EX system automated stainer (Ventana-Bio Tek Solutions).
After incubation with permeabilization solution on ice for 2 min, cells were labeled through incubation with labeling solution (containing 2%FITC-12-dUTPFITC-12-dUTPFITC-12-dUTP
After incubation in 10% normal goat serum in PBS at 37 °C for 60 min, cells were labeled with an anti-GFAP antibody diluted in PBS overnight at 4 °C.
Following blocking with 2% bovine serum albumin in PBS-T (blocking buffer) for 30 min, cells were labeled at 30°C for 2 h with the first antibodies diluted in blocking buffer.
The bilayers were washed with neutral pH buffer (50 mM HepesNaOH pH 7.4, 137 mM NaCl and 0.2 mM EDTA) flowing at 0.05 ml/min for 2 min. They were labeled with fluorescent-labeled streptavidin (30 µg/ml; Invitrogen) for 5 min then washed again with neutral pH buffer.
Thereafter, a multi-frame SPECT acquisition consisting of six subsequent scans of identical bed position was initiated 5 min PI, with a scan time of 10 min for each time frame and a total acquisition time of 60 min. Frames were labeled with their mean acquisition time point, e.g., '10 min' for 5 to 15 min SPECT acquisition.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com