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The results showed that the percent recovery of verapamil after the 10 min interval was always less than that after the next 20 min period of perfusion.
The observed flow in each 10 min interval was then converted to vehicles per hour.
A 3 min interval was left between each tastant application to ensure that the cells were not desensitized as a result of the previous application of tastants.
The slope of the initial linear portion of the curve (over a 15 min interval) was determined using Prism 3.0 (GraphPad Software Inc .. Standard curves were generated using AMC in the presence of the compounds tested, and the amount of AMC liberated per unit time was calculated.
Fluorescence (excitation 380 nm, emission 460 nm) was measured every 30 s for 40 min. The slope of the initial linear portion of the curve (over a 15 min interval) was determined using Prism 3.0 (GraphPad Software Inc .. Standard curves were generated using AMC in the presence of the compounds tested, and the amount of AMC liberated per unit time was calculated.
The mean of 2 consecutive trials with a 5 min interval was calculated as a measure of nocifensive behavior to a cold stimulus.
Similar(52)
A set of ten measurements with a 2 min interval were obtained at room temperature in which thermal dependence of the MMI-DWF is not noticeable.
Tumour growth doubling times after 'the IR treatment alone' and 'the combination with +1 min interval' were '26.5±5.4 days' and '36.5±5.5 days', respectively (Table 1; P<0.05).
Note that consistency of SUV measurements (in-house and when compared to the literature) depends on strict observance of the uptake time, and therefore a 60 min interval is recommended with an acceptable range of 55 – 75 min [ 30 ].
In addition, physiology (EEG and EOG) and KSS (in 5 min intervals) was acquired.
At 1 h after context testing, the mice were placed into a different, white Plexiglas chamber for 3 min and then the tone was turned on for 3 min. Freezing during the first and subsequent 3 min intervals was recorded.
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