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The number of writhes (characterized by contraction of the abdominal musculature and extension of the hind limbs) was then counted at 5 min interval for 30 min.
The maximum tsunami height is searched with 1 min interval for 120 min from the initial rupture of the N2 segment.
During the HVS load application, dynamic strain data from the tested slab were recorded with every 15 min interval, for 30 s each time, and at a sampling rate of 800 Hz for each strain gage.
An initial 3 min exposure period was observed for mosquito behaviour followed by observations after every 10, 20, 30, 40, 50 min, 1 h and after that a small 3 min interval for every 30 min up to 6 h.
Furthermore, to most effectively use the allowable 488 nm excitation dosage, we imaged Ca2+ at short (5 minutes) time intervals to capture the transient behavior of Ca2+ expected during the 0 to 8 hour time window as found in the experiments in which only Ca2+ was imaged at 5 min interval for 20 hours.
The amount of the coronary heart perfusate was collected and measured at 1 min interval for 15 min.
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Body temperature, salivation, and tremor were assessed before the treatment and at 15 min intervals for 120 min after the injection.
Satiety ratings were collected for 20 min intervals for 220 min during the morning testing session.
Total activity, oxygen consumption (O2) and carbon dioxide (CO2) production were measured with a LabMaster (TSE systems) at 26 min intervals for 24 h.
After 1.5 h of stabilization, baseline samples were collected into 7.5 μl 0.02 M formic acid (FA) at 15 min intervals for 2 h.
These instruments collected data at 5 min intervals for a period of 42 days until they were crushed by detachment and collapse of the sheet.
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