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At the end of the QC, the flow cell was primed by loading twice 150 μl of a mix of 75 μl Oxford nanopore-running buffer (2X), 72 μl nuclease-free water, 3 μl Fuel mix, and leaving at least a 10 min interval between subsequent loadings of buffer or library.
During control tests, an artificial CSF was perfused at a rate of 20-25 microliter/min for 5-8 min with a 5 min interval between each sample.
The cells were kept on ice during the 5 min interval between the two runs.
Measured value was an average of five replicates at 1 min interval between them.
In each session, two injections of glutamate (Glu, 0.5 M, 0.2 ml) and two injections of saline (0.9 %, 0.2 ml) into the masseter and temporalis muscles, respectively, were undertaken, with a 15 min interval between each injection.
Each test was repeated three times with a 15 min interval between tests [69].
Similar(36)
Tissue lysates were sonicated at amplitude 8 for 10 s, 6 7 times, with 2 min intervals between two consecutive pulses.
Control reaction was recorded twice with 15 min intervals between readings.
Memory acquisition test was performed four times daily (60 min intervals between tests) for 5 days.
Four sets in total were performed, with 3 min intervals between each set.
Three to four sarcomere lengths were tested in each preparation (10 min intervals between each period of repetitive stimulation).
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