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Both methods give consistent results and show that AGuIX uptake saturates at about 1.25 pg per cell after 30 min incubation time for 0.5 mM of AGuIX incubated.
Head space incubator was kept at 110°C for 15 min incubation time and 300 rpm.
In total, 118 plasma subjects were analyzed by using the aptasensor measurement with 1 μL sample volume and 5 min incubation time.
The limit of detection was determined to be as low as 0.01 μg mL−1 (0.075 U mL−1) trypsin after 140 min incubation time.
Variant M1 retained high specific activity (993 U/mg; equivalent to 93% of wild-type activity) and improved thermal resistance (T50 improved by 1.5 °C compared to wild-type at 58 °C; 20 min incubation time), making variant M1 an attractive enzyme for industrial applications.
Results give optimum conditions for the keratinase to release the soluble proteins: pH 8.2, keratinase concentration 0.14% (weight of keratinase lyophilisate/final volume) and substrate concentration 5% (weight of nail powder/final volume) for nails; temperature 38.8 °C, pH 9, substrate concentration 5% (weight of hoof powder/final volume) and a 5 h 55 min incubation time for hooves.
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Thin line: after 240-min incubation time.
Dashed spectrum is collected after 270-min incubation time.
e Concentration dependent inhibition of [18F]Pyricoxib uptake (60-min incubation time) into HCA-7 cells.
d Blocking of [18F]Pyricoxib uptake (60-min incubation time) into HCA-7 cells using high concentrations (0.1 mM) of different selective COX-2 inhibitors.
Cellular uptake experiments using [18F]Pyricoxib (300 kBq/mL; specific activity >40 GBq/μmol) were performed in triplicates in Krebs buffer at 37 °C with 5-, 15-, 15-, 30-, 60-, 90-, and 120-min incubation time.
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