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After cooling down to the room temperature, purification was accomplished by ethanol, through centrifugation at 10,000 rpm for 12 min, in two stages consecutively [17].

Parameters such as extraction temperature and pressure were investigated by a fractional factorial design (FFD) and the selected extraction (60 °C, 1500 psi for 10 min in two cycles) was most effective.

Logan graphical plots showed a linearity after 50 min in two subjects (NC1 and NC4) but a slight increase of the slope over time in one subject with a possibly lipophilic plasma metabolite (NC2) (Fig. 4a).

Briefly, 200 mg of bulk C60 was stirred in 500 mL of ultrapure water (Millipore Simplicity 185, Merck, Germany) for 10 min at 550 rpm (Rotamix 550 MMH, Tehtnica, Slovenia) and sonicated (Sonorex Super RK 100 H, Bandelin, Germany) for 60 min in two cycles with 30 min of cooling between cycles.

Symbiodinium culture Z4 isolated from the coral Acropora millepora at Nelly Bay, where it has not been observed with WS signs at this site, was the least affected (∼3%; p<0.01) of all Symbiodinium cultures tested in this study [mean I (Z4 ΔF/Fm') = 0.034±0.019 following exposure to P1 supernatant for 10 min in two independent experiments].

For Symbiodinium culture Z1, inactivation (I) of PS II (measured as light adapted quantum yields) was greater than 95% (mean I (Z1 ΔF/Fm') = 0.968±0.016) following exposure to P1 supernatant for 10 min in two independent experiments, and total PS II inactivation resulted after 20 min (Fig. 1A).

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Similar(45)

The longest attack duration was more than 30 min in four patients (24%).

The second user subsequently used them for 5, 30 or 120 min in three distinct simulation sessions.

In this study we administered GTN 0.5 μg/kg/min i.v. for 20 min in six healthy volunteers.

The extraction has been carried out at 100 °C and at an extraction pressure of 1500 psi for 5 min in four extraction step.

Reduction of the shoulder dislocation was achieved within 5 min in 20 patients (64.5%) and within 10 min in eight patients (25.8%).

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