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After the solution is adjusted to pH 7, 500 to 1,000 MBq pertechnetate is added followed by incubation for 5 min in normal atmosphere.
In the optimized labeling protocol, 18F-FDG-labeled RBC PET revealed high retention of the tracer in the cardiovascular system for at least 120 min in normal rats.
We have not found a scenario that also predicts faster rising time toward the PG peak, i.e., a mechanism that enhance the accumulation rate of the cloud charges (2 5 min in normal condition) when the surrounding radioactivity is enhanced.
After an incubation period of 30 min in normal Tyrode's solution, the concentration-response curves (CRCs) of Ca++ were constructed.
The slices were then incubated for 10 min in normal ACSF containing 5 μg/ml propidium iodide (PI) and 20 μM Newport Green (Invitrogen).
Briefly, for mitochondrial stainings, cells were incubated for 30 min in normal growth medium containing 5 μM MitoTracker (Mol. Probes, Inc., Stockholm, Sweden).
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Scratches were made to monolayers, and the cells were incubated at 37°C for 20 mins in normal media.
Sections were treated with 3% hydrogen peroxide for 20 min, blocked in normal horse serum (NHS) for 1 h, and treated with the Avidin Biotin blocking kit (Vector Laboratories).
Nonspecific antibody binding was blocked by 20 min immersion in normal goat serum (20% in PBS).
After 30 45 min incubation in normal ACSF at 36°, the slices were used for recording.
These data rule out significant label loss by lactate exchange in 5 10 min experiments in normal adult rat brain (Dienel and Cruz, 2009).
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