Sentence examples for min in ice from inspiring English sources

Thereafter, it left for 30 min in ice.

The resuspended cell solution was lysed by sonication in short pulses of 15 s for 10 min in ice water bath.

The radionuclide incorporation reaction was halted by cooling the mixture for 2 min in ice and through the addition of 50 nmol diethylenetriaminepentaacetic acid (DTPA, Erasmus MC Pharmacy) to chelate any unbound or "free" 213Bi.

Briefly, after centrifugation for 5 min at 400× g, 1×105 cells were fixed with 2% paraformaldehyde in Tris Buffered Saline solution (TBS) for 20 min and permeabilized with 0.1%TRITON-X-100 and 0.1% Sodium Citrate in TBS for 3 min in ice.

Samples were incubated for 10 min in ice and centrifuged for 10 min at 12,000 g, 4°C.

Twenty four hours after initiation of differentiation cells were fixed for 30 min in ice cold 70% ethanol.

For the methanol pretreatments, the coverslips were fixed for 5 min in ice-cold methanol or 3.7% formaldehyde at room temperature after permeabilization and before the extract was added.

Aliquot A was placed for 10 min in ice-cold water bath, centrifuged at 4 °C, and the resulting plasma was used as comparison sample.

Plants were harvested and roots were washed for 10 min in ice-cold 5 mM CaCl2 solution to displace extracellular Cd (Rauser 1987), rinsed in distilled water and gently blotted with paper towels.

Following incubation, slices were washed twice for 5 min in ice-cooled buffer, dipped in ice-cooled distilled water and finally dried under a stream of cold air.

All slides were then washed (4×15 min) in ice-cold binding buffer, rinsed in ice-cold distilled water and air-dried.