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Exact(4)

To answer the question to what extent a change of the boundary layer characteristics is able to influence the results in terms of local pressures, global forces and responses of the structure, large sample sizes of 240 individual events have been recorded each corresponding to approx. 10 min in full scale.

Pretreatment with inhibitors was performed for 15 60 min in full MEM (as used for RK13 cells and present in EV supernantants).

All seeds were scarified with a razor blade, then surface sterilized for 30 sec in 95% ethanol, followed by 7 min in full bleach.

Cells were pretreated with inhibitors for 15 60 min in full DMEM; EVs (IHD-J F13 GFP, 0.25 ml 35 × concentrated supernatants) or MVs (IHD-J EGFP A5, 7.5 × 10 p.f.u. in 0.25 ml) in serum-free medium were bound to cells on ice for 1 h.

Similar(56)

The film was developed for 12 min in full-strength Kodak D-19 developer and fixed for 8 min in Kodak Rapid Fix.

However, studies concerning WI in porcine kidneys have demonstrated that a WI of up to 120 min results in full recovery of renal function [24].

Fermentation and proofing were carried out at 30 °C for 1 h, while baking was performed at different temperature levels (from 170 to 210 °C) and baking times from 45 to 135 min, in a full factorial design.

For immunofluorescence, cells were washed in pre-warmed full medium and fixed for 15 min at 37°C in full medium containing 3.7% formaldehyde.

Reduced and oxidized control lysates were obtained from cells treated with 10 mM DTT (dithiothreitol) or 5 mM diamide (both Sigma) for 5 min at 37°C in full growth medium.

The total time of scan was 90 min and was acquired in full list mode, similar to the protocol for the control scans described in "MicroPET 18F-nifene studies".

Antigen retrieval for CD8 was performed by microwaving in 1 m M EDTA buffer, pH 8, for 5 min at full pressure in aplastic pressure cooker in a microwave oven.

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