Sentence examples for min in fragmentation from inspiring English sources

Briefly, 500 ng of total RNA isolated from primary keratinocytes was poly-A purified (Dynabeads, Thermo Fisher Scientific, Waltham, MA) and chemically fragmented by treatment 95°C for 8 min in Fragmentation Buffer (Thermo Fisher Scientific).

Next, twenty micrograms (20 μg) of the in vitro transcription product was fragmented by placing at 94 °C for 35 min in fragmentation Buffer.

Ten microliters of purified cDNA were used for the in vitro transcription (IVT) amplification reaction, in the presence of biotinylated nucleotides (Enzo Biochem Inc .. Labeled cRNA (15 μg) was fragmented by incubation at 94°C for 35 min in fragmentation buffer (GeneChip Sample Cleanup, Qiagen) and hybridized competitively against the Affymetrix HG-U133 microarray set.

Isolated RNA was enriched for mRNA by using oligo dT Dynabeads (Invitrogen) according to the manufacturer's instructions and fragmented into fragments of 200 700 nucleotides by incubating at 70°C for 15 min in fragmentation buffer (Ambion).

cRNA, 15 μg per sample, was heated at 80ºC for 33 min in fragmentation buffer (Affymetrix, Inc., Santa Clara, CA).

The cRNA target (20 μg) was incubated at 94°C for 35 min in fragmentation buffer (Tris, MgOAc, KOAc).

For amplification-based sequencing, messenger RNA (2 µg) was fragmented at 85°C for 5 min in a fragmentation buffer (Ambion) and converted to single stranded cDNA using SuperScript II reverse transcriptase (Invitrogen), followed by second-strand cDNA synthesis using Escherichia coli DNA polymerase I (Invitrogen).

The biotin-labeled cRNA was purified using RNeasy mini-column (RNeasy kit; Qiagen, Valencia, CA) and fragmented at 94°C for 35 min in 1X fragmentation buffer (40 mMTris-acetate, pH 8.0, 100 mM KOAc, 30 mM MgOAc).

Following purification, the mRNA was fragmented into smaller pieces at 70°C for 5 min in the fragmentation buffer (Ambion) and reverse-transcribed to synthesize first strand cDNA using SuperScript III reverse transcriptase (Invitrogen) and N6 random hexamers (Takara).

We fragmented 15  μg of cRNA at 94°C for 35 min in a fragmentation buffer containing 40 m M Tris-acetate (pH 8.1), 100 m M potassium acetate and 30 m M magnesium acetate.

Fragments between 35 200 bp in length were generated by incubating the cRNA at 94°C for 35 min in a fragmentation buffer.