Sentence examples for min in darkness from inspiring English sources

Then, 100 μl 0.05 M iodoacetamide in UA buffer was added to block the reduced cysteine residues, and the samples were incubated for 20 min in darkness.

Light reduced the latency to sleep from ~30 min in darkness (baseline) to ~10 min at the highest intensity, although this effect was invariant across the light intensities used.

Solution was incubated for 10 min in darkness.

The reactive mixtures were incubated at 37 °C for 30 min in darkness.

The cell nuclei were then stained by Hoechst 33342 for 10 min in darkness.

Before irradiation, the samples were put in 40-mL MB for 30 min in darkness to reach absorption equilibrium.

The cells were then stained by Hoechst 33342 for 10 min in darkness and fixed with 4% paraformaldehyde for 30 min.

For digestion, the protein solution was reduced with dithiothreitol (DTT) (5 mM) for 30 min at 56 °C and alkylated using iodoacetamide (IAA) (20 mM) at room temperature for 45 min in darkness.

The cells were stained with Lysotracker Green (100 nM) for 30 min and Hoechst 33342 (10 μg/mL) for 10 min in darkness to visualize the lysosomes and nuclei, respectively.

After 30 min adsorption in darkness, the adsorption-desorption is at an equilibrium between photocatalyst and RhB molecules.

The mixture was incubated for 30 min in total darkness and the absorbance was read at 517 nm.