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The game's chat window displayed a textual record of the blows landed and the cost to Min in damage points.
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More recent studies by others using the murine model, and BCCA occlusion for 22 min resulted in damage in the hippocampal area as well as the striatal area (Yoshioka et al., 2011b).
Furthermore, the selective ERK1/2 inhibitor PD98059 (Cell Signaling, Danvers, MA, USA) was injected intracerebroventricularly (2 μl; 200 μM) 30 min before damage in SCH-sal group.
As the 20 min exposure led to a higher variability in damage severity, we used a 30 min exposure for all further experiments.
It was concluded that continuous loading for >40 min causes creep damage in bovine TMJ disc, and that prolonged sustained tension affects the recovery of joint homeostasis.
As a positive control, cells were heat damaged by 45°C for 30 min in the presence of 10% methanol.
Additionally, HNO3 gave superior removal efficiency of 90% in only 50 min without damaging the peels.
When cells were incubated with QDs (0.05 μM) or AlPcS (5 μM) for 50 min, no detectable damage could be found in these cells without light irradiations.
Zhu Min in 2008.
Sections were deparaffinized 2×5 min in xylene.
for 3 min in 1 ml Trizol.
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