Sentence examples for min in complete from inspiring English sources

Briefly, cells grown on coverslips were incubated with lysotracker 1∶20,000 for 20 min in complete medium and washed three times.

Under the same conditions, only about a tenth of the population had duplicated the chromosomal ori locus (Figure 4B), whereas after 30 min in complete media, 57% of the swarmers showed fully or partially segregated origins (Figure 4C).

To follow receptor-mediated endocytosis, cells were incubated on ice for 20 min with 1.3 nM Fluorescein-M-hMPV, washed twice at 4°C, and then incubated at 37°C for 5 to 30 min in complete RPMI medium.

The cells were incubated at room temperature for 5 min in complete medium containing 2.5  μmol/l CFSE.

To visualize lysosomes, the cells were stained with LysoTracker Red (500 nM) for 30 min in complete medium and were washed free of dye prior to fixation.

Cells were rinsed twice with 0.2 M PBS (pH 7.4) and incubated for 10 min in complete culture medium containing 0.5  μg/ml FITC-conjugated Annexin-V and 2  μg/ml PI.

For instance, sonophotocatalytic treatment at 400 W UVA power with H2O2 for 120 min resulted in complete mineralization followed by significant toxicity reduction.

As shown in Figure 2A, a pretreatment time even as short as 1 min resulted in complete inhibition of Ag-triggered MC degranulation by Ro5-4864.

However, when placed in PSF, a dynamic transformation takes place during which new LaPO4 peaks can be seen within 15 min, culminating in complete transformation within ∼24 h.

Using freshly prepared sample buffer and boiling of samples for at least 10 min resulted in complete denaturation of the dimer into the monomer.

After repeated washings, the released cells were pelleted (1000 rpm, 5 min), suspended in complete medium, plated at a density of 60 × 106 cells per 150 cm2 tissue culture flask and maintained at 37°C in 5% CO2.