Sentence examples for min in cold from inspiring English sources

The incubated sections were washed twice for 5 min in cold 170 mmol/L Tris HCl (pH 8.2) containing 0.25% BSA.

The incubated sections were washed twice for 5 min in cold incubation buffer containing 0.25% bovine serum albumin, then in buffer alone, and dried quickly.

Seven µm-thick cryosections were fixed for 10 min in cold acetone and rinsed in PBS.

For β-glucuronidase activity in green tissues, RLK902::GUS dissected leaves were collected in microcentrifuge tubes on ice and incubated for 20 min in cold 90% acetone.

Cells were washed three times in 0.01 M Phosphate Buffered Saline (PBS), fixed for 30 min in cold 4% paraformaldehyde, and washed again 3 times in PBS.

Mice doubly heterozygous for BAT-gal and an oto mutation were crossed to oto heterozygotes; resulting embryos were fixed for 5 min in cold 4% PFA, rinsed, stained overnight at room temperature with Xgal, and post-fixed.

Cells were fixed in 4% paraformaldehyde in PBS for 20 min followed by treatment with 50 m m NH4Cl for 10 min or in cold 20% methanol for 10 min (for occludin staining).

Nuclei were prepared by two 500 g centrifugation steps, each 5 min, separated by a 5 min wash in cold PBS.

Seven types of stressors were used (40-h water deprivation, 40-h food deprivation, revered light dark-cycle, 5-min hot environment, 5-min swimming in colight dark-cycle cage shake and wet cage) in a semi-random order.

48 hrs post-transfection, coverslips were washed in PBS and fixed 10 min. in cold methanol followed by 10 min. in cold acetone.

Cells were fixed with 4% paraformaldehyde on ice for 15 min, followed by washes with PBS and 5 min permeabilization in cold methanol.