Sentence examples for min in buffered from inspiring English sources

MARY-X spheroids, tumor emboli and MCF-7 agarose-induced spheroids were fixed for 2-24 hours at 4°C in 3%Glutaraldehyde/0.11 M sodium cacodylate buffer (pH 7.2) and post-fixed for 45 min in buffered 1% osmium tetroxide.

Cells were plated on CC2-treated Lab-Tek™II 4-well chamber slides (Nalge Nunc, Rochester NY), washed with PBS, fixed for 10 min in buffered formalin, and permeabilized in 2% BSA, 0.2% Triton-X 100, PBS.

A replica gel containing xylan (0.5%) was incubated at 50°C for 5 min in buffer and then stained in 0.1% Congo Red solution.

EUDRAGIT® L 30 D-55 single-coated tablets showed a slow drug release with a lag time of 75 min in buffer, whereas release from the EUDRAGIT® L 30 D-55 double-coated tablets was accelerated.

After several washes, immune complexes were recovered by heating the samples at 95°C for 10 min in buffer containing SDS.

Embryos (E10.5 E12.5) were washed in unadulterated buffer and then fixed for 30 45 min in buffer containing 0.2% glutaraldehyde, 5 mM EGTA, and 2 mM MgCl2.

After isoelectric focusing, the IPG strips were equilibrated for 15 min in equilibration buffer I [6 M urea, 2% (w/v) SDS, 0.05 M Tris-HCl (pH 8.8), 20% (v/v) glycerol and 2% (w/v) dithiothreitol (DTT)] followed by 15 min in buffer II (same as buffer I but containing 2.5% iodoacetamide instead of DTT).

The homogenate was stirred for 30 min in buffer P without CHAPS at 4°C in the presence of 0.025 parts v/w of benzonase (Merck, Darmstadt, Germany) and a final concentration of 5 mM magnesium chloride in the sample.

To examine the surface barrier function, living worms were incubated for 15 min in buffer containing a nuclei-staining dye Hoechst 33342, which does not usually permeate the worm cuticle barrier.

To determine the complex I activity, submitochondrial fractions (0.6 mg/ml) were incubated for 3 min in buffer solution containing 250 mM sucrose; 50 mM potassium-phosphate; 1 mM KCN; 50 µM decylubiquinone; 0.8 µM antimycin, pH 7.4.

The optimal pH of Xyn11B for enzyme activity was determined at 40°C for 10 min in buffers mentioned above.