Sentence examples for min in buffer from inspiring English sources

For second-dimension electrophoresis, IPG strips were equilibrated for 10 min in buffer I [37.5 mM Tris-HCl (pH 8.8) containing 6 M urea, 2% w/v SDS, 20% v/v glycerol, and 2% DTT] and then re-equilibrated for 10 min in buffer II [same buffer containing 2.5% iodoacetamide in place of DTT].

EUDRAGIT® L 30 D-55 single-coated tablets showed a slow drug release with a lag time of 75 min in buffer, whereas release from the EUDRAGIT® L 30 D-55 double-coated tablets was accelerated.

A replica gel containing xylan (0.5%) was incubated at 50°C for 5 min in buffer and then stained in 0.1% Congo Red solution.

Embryos (E10.5 E12.5) were washed in unadulterated buffer and then fixed for 30 45 min in buffer containing 0.2% glutaraldehyde, 5 mM EGTA, and 2 mM MgCl2.

After several washes, immune complexes were recovered by heating the samples at 95°C for 10 min in buffer containing SDS.

To examine the surface barrier function, living worms were incubated for 15 min in buffer containing a nuclei-staining dye Hoechst 33342, which does not usually permeate the worm cuticle barrier.

The optimal pH of Xyn11B for enzyme activity was determined at 40°C for 10 min in buffers mentioned above.

The precipitate was washed 4 times with CHAPS buffer and boiled 5 min in loading buffer before immunoblot analysis.

Membranes were washed (two 10-min washes) in washing buffer and equilibrated (2 5 min) in detection buffer (0.1M Tris-HCl, 0.1m NaCl, pH 9.5).

Mn2+ that was present following Pi-affinity PAGE was removed prior to electroblotting by incubating the gels for 10 min in transfer buffer containing 1 mM EDTA, and then for 10 min in transfer buffer lacking EDTA.

Gels were incubated for 30 min in denaturation buffer (1.5 M NaCl, 0.5 M NaOH) and then for another 30 min in neutralization buffer (1.5 M NaCl, 0.5 M Tris, pH 7.5).