Sentence examples for min in absolute from inspiring English sources
Next day, sections were pretreated for 3 min in absolute alcohol, followed by 1 min in 70% ethanol and 1 min in distilled water.
In brief, after de-paraffinisation, endogenous peroxidase activity was quenched by immersing the sections for 30 min in absolute methanol containing 0.3%H2O22.
Cells were incubated 7 min in 2× SSC and finally dehydrated by 10 min incubation in 70% ethanol followed by 10 min in absolute ethanol.
Endogenous peroxidase activity was quenched by incubating the slides for an additional 30 min in absolute methanol and 3% hydrogen peroxide.
Glass microscope slides were prepared by soaking in hexane 20 min, then 20 min in absolute ethanol followed by drying 5 h at 90°C.
Tissue cryosections (14 μm thickness) on poly- L-lysine-coated slides were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 30 min at room temperature, washed three times with PBS for 10 min each time, dehydrated for 2 min in absolute ethanol and then treated with permeabilisation solution (1% Triton X-100 in 1% sodium citrate) for 15 min at room temperature.
Following this step, the slides were neutralized in 0.4 M Tris-HCl (pH 7.5) for 15 min, fixed in absolute ethanol for 5 min, and stored at room temperature until analysis.
Fixed materials were dehydrated through a 20% increment of ethanol concentration every 15 min from 30%to90%0%, followed by two subsequent dehydration steps of 15 min each in absolute ethanol.
Briefly, the deparaffinized sections were treated with 20 μg/ml proteinase K for 15 min and immersed in absolute methanol containing 0.3%H2O22 for 10 min at room temperature to block endogenous peroxidase activity.
In the TNK/ENOX group achieving complete ST resolution by 180 min, in-hospital reinfarction was 1.9% vs 4.2% for TNK/UH (P=0.015) representing a 2.3% absolute and 55% relative reduction in reinfarction.
The slides were then rinsed in 2x SSC for 5 min and dehydrated in absolute ethanol and allowed to air dry.
