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Briefly, the gastrocnemius and tibialis anterior muscles were harvested and incubated for 30 min in a digest medium (Thermo, #17703034).
Lysates were sonicated to obtain chromatin fragments <1 kb and centrifuged for 15 min in a microfuge at room temperature.
The fibroblasts were incubated for 30 min in a humidified CO2 incubator and harvested for western blotting and immunofluorescence assay.
Maple (Acer rubrum) wood chips were pyrolyzed at 500 °C for 30 min in a modified muffle furnace28.
The extract was clarified by centrifugation at maximum speed for 10 min in a tabletop centrifuge at 4 °C.
The LAMP assay was performed at 62 °C for 60 min in a water bath.
Detection from sample application to final results was completed in 6 min in a reagentless process.
Thereafter, the female's behavior was monitored for 20 min in a male preference procedure.
The gelation time was significantly short (∼10 min) in a low concentration of TEA (4 mM).
"How many did he give you to eat, again?" 31 min: In a radical departure from normality, Ireland are going all-out attack now.
Data acquisition was performed over 60 min in a 3D list mode.
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