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The multistage, single-cell assay reported here offers a throughput in the electrophoresis stage (5 min handling time) of ∼30 cells/min demonstrated for 153 cells total.
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In this study, precooling (i.e., lowering the initial temperature) and ice bath cooling (i.e., lowering the surrounding temperature) prolonged the handling time to 15.2 min and to 112.5 min, respectively.
They were set free after data-loggers were attached to the feathers of the bottom part of the back, using Tesa® tape secured with cyanoacrylate glue (Loctite® 401) (handling time: 10 min max).
Compared to the control lacking of xylanase, the dough handling time reduced from 6.5 min to 6.0 min and the height-diameter ratio of dough increased with the increasing concentration of XYNZG.
The handling time of all containers is 1857 min, which is 31.0 h.
The handling time was generally less than 10 mins.
This method further allowed us to minimize the handling time, attaching and retrieving the logger in less than 5 min before birds were returned to their nest-boxes.
3.42pm GMT 55 min Replacements time.
Title: Lucky Kiwi and Coconut Cupcakes Author: Adapted from Foodnetwork Prep Time: 1 hr and 10 min Cook Time: 25 min Total time: 1 hr 35 min Serves: 20.
77 min: "Crazy shot time!
45 min: Not this time.
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