Sentence examples for min gradient were from inspiring English sources

Blank injections using a 30 min gradient were performed between each sample.

Duplicate LC-MS runs for each well injecting ∼2 μg of the digested peptide mixture with a simple 90 min gradient were performed, defined here as LC-MS replicates.

A proteome sample prepared from C. cellulovorans was injected onto the column, and a (A) 600 or (B) 60 min gradient was applied with a flow rate of 500 nL/min.

A 95 min gradient was performed with a 15 min washing period (100% A for the first 10 min followed by a gradient to 98% A at 15 min) to allow for solid phase extraction and removal of any residual salts.

The following gradient profile was applied: After 1 min of isocratic run at 99% A a linear 12 min gradient was applied to 65% A immediately followed by an 1.5 min gradient to 30% A and a 1 min gradient to 1% A. Then a 1.5 min isocratic period at 1% A followed before switching back to 99% A to re-equilibrate the column for 2.5 min before to the next sample was injected.

A 150 min gradient was used for the 1D-LC/MS analyses.

A 20 min gradient was employed with a 10 μL injection volume, and the flow rate was 15 μL/min.

A 5 min gradient was applied and elute concentration was then switched to 55% (v/v) water and 45% (v/v) 100 mM NaOH for 30 min.

They also show that while the Min gradient is independent of the chemical nature of the lipid bilayer, geometric cues play a rather critical role in its patterning.

A 50 min gradient was employed, which consisted of a 35 min gradient from 2 40% acetonitrile with 0.1% (v/v) formic acid, after which, the acetonitrile increased to a maximum 80% for the remainder of the run.

To address how downstream processes of the Min gradient are affected by the length dependent oscillation modes in compartments of different length, we investigated FtsZ-mts localization in these compartments.