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The product was eluted around 22 min for total running time per sample.
Robot console times and total operative times were consolidated after approximately 50 cases at about 50 min for console time and 90 min for total operative time [34].
Individual tremor bands lasted 25 35 min and quiescent spacings between bands 140 160 min, for total cycle durations of 165 195 min.
Mice were assessed for a period of 60 min for total activity, mobility and rearing and the P-values obtained through the analyses are displayed in Table 2 for each parameter.
For this we used 500ng of total RNA and 4.5µl of RT reaction mix in a total volume of 7.5µl at the following cycle conditions: 16°C for 2 min, 42°C for 1 min and 50°C for 1 min for total of 40 cycles followed by 85°C for 5 min and bringing the contents to 4°C.
Images were taken every 20 min for total movie duration of 12 hr.
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Bouts of scratching directed toward the injection site were counted in 5 min intervals for total 30 min.
After 5 min, cells were stimulated with or without 20 nM CXCL12 for 5 min. To analyse T cell polarization, cells were fixed with 4% paraformaldehyde for 10 min. For total RhoA or phospho-ERM localization, cells were fixed with 10% trichloroacetic acid for 15 min on ice.
From 20 g of a crude extract of licorice roots, 2.2 g of GL were recovered after 70 min, for a total process duration of 90 min.
The system was kept at 10 90 for 5 min and then re-equilibrated to 95 5 in 10 min for a total of 65 min run cycle.
The oven temperature program is set to hold 85 °C for 2 min, followed by a ramp to 280 °C at 4 °C/min which is held for 20 min for a total run time of 70.75 min.
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