Sentence examples for min for tissue from inspiring English sources

Using minimal and maximal inter quartile values for these time constants leads to a [AUCNIF/λCpN tpeak)] ratio of 272 and 262 min, for tissue activity peak at 82 and 76 min, respectively.

Permeabilization was made with 0.1% Triton X-100 in PBS for 30 min for cultured cells and 60 min for tissue sections, at room temperature (RT).

The 0.05% diaminobenzidine tetrahydrochloride (DAB) was used for 15 min for tissue stain and finally washed twice by the Tris buffer [ 8, 11].

To enhance entry of PCR mix into cells, samples were made permeable by digestion with 2 mg/mL pepsin in 0.1 N HCl (40 80 min for tissue sections; 20 min for control cell smears), pepsin inactivation solution (100 mmol/L Tris-HCl, 100 mmol/L NaCl, pH 7.4) applied for 1 min, followed by a DPBS rinse and 5 min in absolute ethanol.

Optimum retrieval time was determined empirically to be 40 min for whole tissue sections and 30 min for TMAs.

This time increased to 10 min for plant tissues, which require mechanical disruption.

The samples were then centrifuged at 10,000 rpm for 10 min at 4 °C for tissue samples and at 2000 rpm for 10 min at 4 °C for plasma samples.

After a 10-min transmission scan for tissue attenuation correction, infusion of [11C]PIB (about 370 MBq in 5 mL for 1 min) began.

After incubation on ice for 20 min, tissue lysates were clarified for 10 min at 12,000 × g at 4°C, denatured at 95°C for 5 min, and stored at -80°C until needed.

After drying, the sections were rehydrated in PBS for 5 min. Tissue sections processing for immunofluorescence using the polyclonal mASAP rabbit serum (used at a 1/500 dilution) were as described [ 67].

Immunostaining cells cultured on chamber slides were rinsed in PBS and fixed in ice cold acetone for 5 min. For frozen tissue samples tumor tissue was fresh-frozen in Tissue-Tek O.C.T. compound (Sakura Finetechnical, Tokyo, Japan), cryosectioned at 10 um then fixed in acetone for 10 min at -20°C.