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Figure 2 Absorption versus time (min) for red marble (RM1).
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Figure 3 Absorption versus time (min) for dark red marble (RM2).
A typical Turkevich synthesis of AuNPs at 373 K takes 20 min for the characteristic red wine color to appear in the solution.
a RMS amplitude over 1 min for the microphone (red line) and the vertical ground velocity (black line) at the OWD station during the period from 07:00 to 11 00 June 29 , 2015and b a temporal sequence of the microphone seismometer correlation function of the band-passed seismic and infrasonic signals.
Blood was mixed with a dextran solution (3% (w/v) Dextran 500 in 0.9% NaCl, both Sigma Aldrich) by gentle inversion and left at room temperature for 20 min for sedimentation of red blood cells.
2 mL of 5% FeCl3 solution and 50 mL volume of distilled water was added to 1 mL of colourless solution and kept for 30 min for colour (blood red) development.
For immunophenotyping analyses, blood aliquots containing 106 leucokytes were incubated for 10 min with PharM Lyse (BD) for red blood cells lysis, followed by an 30 min incubation with anti-CD3/FITC, anti-CD4/PE, anti-CD8/PE, anti-CD11b, anti-CD16/32 (Fc block), anti-CD19/PE, anti-CD45 and anti-Pan-NK/PE.
After 2 weeks differentiation induction, cells were fixed with 4% formaldehyde solution for 30 min; then, alizarin red stain for osteogenic differentiation and oil red stain for adipogenic differentiation were carried out.
The time-lapse images were collected every 30 sec, for 15 min for both green and red wavelengths using the Metamorph software.
Incubation times usually range between 5 and 15 min for both the Nile red and BODIPY 505/515.
FACS lysing solution was added into each tube and incubated in dark for 20 min for the lysis of red blood cells.
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