Sentence examples for min for primary from inspiring English sources

Both the rate and length of transients remained unchanged at all timepoints studied up to 20 min for primary cardiomyocytes exposed to extracts from the GBS Δβh/c mutant (Fig. 3A C) or with buffer alone (data not shown).

The epidermal sheets were incubated with shaking in a trypsin solution (0,25% Tryp/EDTA 250 µM/PBS) at 37°C for 15 min for primary cultures and for a second 15 min for analyses.

Incubation times were 90 min for primary.

The immunoprecipitates and whole cell lysates were subjected to SDS-PAGE, transferred to Immobilon-P membranes and the association of RPTOR with MTOR determined using western blot techniques as described above., Freshly isolated lenses, lenses from organ culture, and primary lens cell cultures were fixed in 3.7% formaldehyde (15 min for primary cultures and overnight for whole lenses).

Amplification was carried out in a BiometraTM PCR programmed for 40 cycles as follows: 94 °C/4 min for primary denaturation (1 cycle); 94 °C/1 min for denaturation, 55 °C/1 min for annealing with bar gene primers and 58 °C/1.2 min for annealing with Avidin gene primers, 72 °C/2 min for extension (38 cycles); 72 °C/8 min (1 cycle); 4 °C (overnight storage).

Therefore, we set 20 min for the primary immunoreaction and 30 min for the secondary immunoreaction as the optimized immunoreaction time.

Cells were harvested, washed in FACS buffer and incubated with antibodies (4 °C, 30 min for both primary and secondary antibodies).

Based on these assumptions, the sample size required to detect the MDI (45 min) for the primary outcome (workplace sitting, assumed SD = 70, pre-post correlation = 0.4) is 160 per group, spread across 8 clusters each.

Thermal cycling parameters were: 2 min at 50° and 10 min at 95° for primary denaturation, followed by 40 cycles of 15 s at 95° and 1 min at 60°.

The mixtures were incubated for 4 min at 94oc for primary denaturation, 60 sec at 94oc for secondary denaturation of the target DNA and then, annealing at 50oc for 1 min, and extension at 72oc for 1 min that 35 cycle was performed.

For antigen retrieval, sections were either heated in citraconic anhydride (pH 7.4; 45 mins at 100°C) or immersed in protein kinase 20 μg/ml (20 mins at 37°C; for primary antibody B7 only).