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Assuming a half life of ≈20 min for plasma melatonin [39], it seems that the values found in the in vitro system (as expressed per day in Fig. 2) are within the range of those measured in the plasma of the implanted animals.

Additional patient time was also considered: 10 min for capillary testing and 30 min for plasma testing.

Blood samples were taken at 0, 30, and 60 min for plasma glucose assay in order to determine the optimal dose to induce the hypoglycemic effect.

From 1400 h, venous samples were obtained every 15 min for plasma glucose concentration measured by the Yellow Springs Instrument analyzer (YSI2300 STAT Plus Analyzer, Farnborough, U.K).

An aliquot of EDTA blood for DNA isolation was removed, and the remaining blood centrifuged at 1500 × g for 10 min for plasma isolation.

Differences in demographic characteristics, in baseline blood glucose, CCK, and PYY concentrations, and in AUC0 120 min for plasma CCK and PYY between critically ill groups were compared using the Student unpaired t test and the chi-square test.

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Similar(52)

The other subsamples were immediately centrifuged (1,500 g, 15 min, 4°C) for plasma separation and the plasma was preserved at −80°C until analysis.

After a 12-h fast, a 75-g OGTT was performed with 0, 30, 60, 90, and 120 min sampling for plasma glucose and insulin.

The mean time to coagulation was 95 min for rabbit plasma and 73 min for horse plasma.

The test line was clearly visible at 10 min for each plasma dilution positive for ICT, whereas negative dilutions displayed no test line, even beyond the 10 min recommended by the manufacturer.

Blood samples were obtained at 7.5-min intervals for measurement of plasma glucose concentration and fractional plasma glucose C enrichment or atom percentage excess (APE), and at 60-min intervals for plasma insulin.

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