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The mixture was shaken for 1 min and centrifuged at 15,000×g for 2 min for phase separation, and the aqueous phase was then retrieved.
Two thermal treatments were applied to the samples: (1) The samples were directly quenched from 230 °C to 132 °C for isothermal crystallization; (2) The samples were firstly held at 160 °C for 60 min for phase separation and then cooled to 132 °C for crystallization.
The vials were vortexed vigorously for 1 min and then centrifuged for 6 min for phase separation.
Afterwards 500 μL 0.45 % NaCl was added, the tubes were shaken briefly, and centrifuged at 2000g for 2 min for phase separation.
After cooling at room temperature, 1 ml of distilled water was added into the tubes; the mixture was shaken vigorously for 1 min and kept for about 30 min for phase separation.
After the addition of 350 μL of cyclodecanone as internal standard (freshly prepared methanolic solution, 20 μg/mL), 1 mL of the sample was shaken with 10 mL of ethanol (15% vol) and 1 mL of 1,1,2-trichloro-1,2,2-trifluoroethane for 60 s and centrifuged at 3.000 rpm for 5 min for phase separation.
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The delay time was 50 s for portal venous phase imaging and 3 min for delayed phase imaging.
It resulted with activation energy of 62 kJ/mol and 138 kJ/mol and pre-exponential factor of 4.3e5 min-1 and 2.25e9 min-1 for phase 2 and phase 3, respectively.
The mixture was vortexed vigorously for 1 min and centrifuged at 10,000 rpm for 5 min for the phase separation.
The reaction mixture was vortexed and centrifuged at 70 × g for 10 min for full phase separation.
The tubes were chilled on ice for 5 min and centrifuged for phase separation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com