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We demonstrate minimal toxicant detection of 10 μg/ml for NA using 30 min for induction and 0.31 μM for IQ using 120 min for induction, both 3 min after the addition of the substrate material.
In this model, the intact uterus containing rodent pups is isolated from anesthetized dam by a C-section on the expected day of birth and immersed in a water bath kept at 37°C for 14 17 min for induction of intra-uterine global hypoxia.
Anesthetized and ventilated rabbits were placed on CPB (α-stat strategy) utilizing a membrane oxygenator with nonpulsatile pump flows of 150-200 ml/kg body weight per min for induction of DHCA by active cooling to 15 °C rectal temperature.
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For chromatin analysis 2 3.5 hr embryos were collected and 20 min heat-shock treated for induction of transgenes as described previously (Li and Arnosti, 2011).
Digital images of Arabidopsis thaliana seedling roots from two independently reared sets of 162 recombinant inbred lines (RILs) and one set of 92 near isogenic lines (NILs) derived from a Cape Verde Islands (Cvi) × Landsberg erecta (L er) cross were collected automatically every 2 min for 8 hr following induction of gravitropism by 90° reorientation of the sample.
Testing with single pulses was resumed for 60 min after induction of synaptic plasticity.
The same dependence of cerebellar LTP on PI3K activity was also observed when LTP was induced with DEA/NO, as preincubation of slices with 1 µM wortmannin for 10 min impaired induction of potentiation by DEA/NO (Figure 4C).
To reach the possible maximum effect, anise oil was applied for 60 min before induction of hypoxia.
HeLa cells were incubated with z-VAD-fmk (10 or 50 μM) for 30 min before induction of apoptosis by 4-h incubation with 1 μM STS.
In vitro analysis of synaptic plasticity is usually conducted for 60 90 min after induction of synaptic plasticity; however, the effects of group I antagonism on synaptic plasticity in vivo typically appear 2 3 h after induction of plasticity (Manahan-Vaughan, 1997).
Where indicated, the cells were treated with BU52 (3 μg/ml), hHA (400 μg/ml) or sHA (6 10 disaccharides, 1 μg/ml) at 37 °C for 30 min before induction, unless otherwise stated.
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